Multiplex RT-PCR Assay for the Detection of Apple stem grooving virus and Apple chlorotic leaf spot virus in Infected Korean Apple Cultivars

The RT-PCR identification and sequence analysis of Apple chlorotic leaf spot virus from apple cultivars in Jiaodong Peninsula, China

A set of specific primer pairs was utilized to detect Apple chlorotic leaf spot virus (ACLSV) from seven different apple cultivars in Jiaodong Peninsula via reverse transcription polymerase chain reaction (RT-PCR), and the sequence of ACLSV genome was analysed. The results indicate that: (1) High-purity total RNA could be successfully isolated using plant RNA rapid extraction kit. The ratios of...

شناسایی مولکولی و گروه بندی جدایه های دو ویروسapple stem grooving virus و apple stem pitting virus در استان اصفهان

چکیده سیب درختی (malus domestica borkh) متعلق به خانواده rosaceae از میوه های مهم و اقتصادی مناطق معتدله و سردسیری ایران است. ویروس ساقه شیاری سیب apple stem grooving virus (asgv) عضو تیپ جنس capillovirus و ویروس ساقه آبله ای سیب apple stem pitting virus (aspv) عضو تیپ جنس foveavirus متعلق به خانوادهbetaflexiviridae ، از مهمترین ویروس های بیماری زای سیب محسوب می شوند. آلودگی این دو ویروس درس...

Molecular Characterization and Diagnostic Development for Apple chlorotic leaf spot virus

The Multifunctional Roles of Apple chlorotic leaf spot virus 50KP Movement Protein

Diverse plant virus families encode movement proteins (MPs) in their genomes. MPs are required for cell-to-cell movement of the virus in host plants and modify plasmodesmata in the cell wall, allowing cell-to-cell movement of virus particles or infectious transcripts. In recent years, much progress has been made in determining cell-to-cell movement functions of MPs from herbaceous plant viruses...

Genome Sequences of Three Apple chlorotic leaf spot virus Isolates from Hawthorns in China

The genome sequences of Apple chlorotic leaf spot virus (ACLSV) isolates from three accessions of hawthorns (Crataegus pinnatifida) grown at Shenyang Agricultural University were determined using Illumina RNA-seq. To confirm the assembly data from the de novo sequencing, two ACLSV genomic sequences (SY01 and SY02) were sequenced using the Sanger method. The SY01 and SY02 sequences obtained with...